Yes. But if you start it up again with the same email address, everything should eventually be reinstalled.
I've rooted my Samsung tablet multiple times, and I had to start over each time (except the current one) because I munged something up. I reused the email address. Everything that was installed was reinstalled. Not immediately, but over the course of a few hours.
That only applies to any apps you get through the Play Store, though. Maybe.
Anything you get from Amazon will need to be manually reinstalled. But anything you got from Amazon will still be marked as something you installed before, so it's not too hard to find. It's tied to your account.
I don't remember if any of the apps from the Samsung store were reinstalled. Probably were because they keep backups of much of what's on the device. But I can't say for sure.